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Bcftools Commands

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bcftools

Bcftools Command: Add Annotated Columns From Tabix Indexed Table

bcftools annotate -a <path/to/annotations.tsv.gz> -c CHROM,POS,REF,ALT,INFO/AF <path/to/input.vcf.gz>
bcftools

Bcftools Command: Add Annotated Columns From Table

bcftools annotate -a <path/to/annotations.tsv.gz> -c CHROM,POS,REF,ALT,INFO/AF <path/to/input.vcf.gz>
bcftools

Bcftools Command: Annotate Vcf

bcftools annotate -a <path/to/annotations.tsv.gz> -c CHROM,POS,REF,ALT,INFO/AF <path/to/input.vcf.gz>
bcftools

Bcftools Command: Concat Vcf

bcftools concat <path/to/chr1.vcf.gz path/to/chr2.vcf.gz ...> -O z
bcftools

Bcftools Command: Concatenate Sorted Vcf Files

bcftools concat <path/to/chr1.vcf.gz path/to/chr2.vcf.gz ...> -O z
bcftools

Bcftools Command: Concatenate Sorted Vcf Files To Zipped Vcf

bcftools concat <path/to/chr1.vcf.gz path/to/chr2.vcf.gz ...> -O z
bcftools

Bcftools Command: Create Index For Bgzipped Vcf

bcftools index <path/to/input.vcf.gz>
bcftools

Bcftools Command: Create Index For Bgzipped Vcf File

bcftools index <path/to/input.vcf.gz>
bcftools

Bcftools Command: Filter Low Quality Variants

bcftools filter -e 'QUAL<20' -s LowQual <path/to/input.vcf.gz>
bcftools

Bcftools Command: Filter Low Quality Variants And Annotate

bcftools filter -e 'QUAL<20' -s LowQual <path/to/input.vcf.gz>
bcftools

Bcftools Command: Filter Variants

bcftools filter -e 'QUAL<20' -s LowQual <path/to/input.vcf.gz>
bcftools

Bcftools Command: Index Vcf

bcftools index <path/to/input.vcf.gz>
bcftools

Bcftools Command: Intersection Vcf

bcftools isec <path/to/a.vcf.gz path/to/b.vcf.gz ...> --threads 4 -o <path/to/intersection.vcf>
bcftools

Bcftools Command: Merge Non Overlapping Samples From Vcf

bcftools merge <path/to/cohort1.vcf.gz> <path/to/cohort2.vcf.gz> --no-index
bcftools

Bcftools Command: Merge Non Overlapping Samples From Vcf Files

bcftools merge <path/to/cohort1.vcf.gz> <path/to/cohort2.vcf.gz> --no-index
bcftools

Bcftools Command: Merge Vcf

bcftools merge <path/to/cohort1.vcf.gz> <path/to/cohort2.vcf.gz> --no-index
bcftools

Bcftools Command: Output Variant Intersection Between Vcf Files

bcftools isec <path/to/a.vcf.gz path/to/b.vcf.gz ...> --threads 4 -o <path/to/intersection.vcf>
bcftools

Bcftools Command: Output Variant Intersection Using Threads

bcftools isec <path/to/a.vcf.gz path/to/b.vcf.gz ...> --threads 4 -o <path/to/intersection.vcf>
bcftools

Bcftools Command: Sort Vcf

bcftools sort <path/to/input.vcf.gz> -O b -o <path/to/sorted.bcf> -W
bcftools

Bcftools Command: Sort Vcf File And Index Output

bcftools sort <path/to/input.vcf.gz> -O b -o <path/to/sorted.bcf> -W
bcftools

Bcftools Command: Sort Vcf File Variants

bcftools sort <path/to/input.vcf.gz> -O b -o <path/to/sorted.bcf> -W
bcftools

Bcftools Command: View Bcf

bcftools view <path/to/input.bcf> -O v
bcftools

Bcftools Command: View Bcf File And Convert To Vcf

bcftools view <path/to/input.bcf> -O v